Elizabeth A. Wood

Position title: Senior Research Specialist

Email: ewood@biochem.wisc.edu

B.A. 1979 University of Wisconsin-Madison
Joined Cox Lab in 1984

Photo of Liz Wood

Henry C, Loiseau L, Vergnes A, Vertommen D, Mérida-Floriano A, Chitteni-Pattu S, Wood EA, Casadesús J, Cox MM, Barras F, Ezraty B. (2021) Redox controls RecA protein activity via reversible oxidation of its methionine residues. eLIFE 10:e63747. DOI: 10.7554/eLife.63747

Jain, K, EA Wood, ZJ Romero, MM Cox. (2020) RecA-independent recombination: Dependence on the Escherichia coli RarA protein. Mol Microbiol. DOI: 10.1111/mmi.14655

Bruckbauer, ST, J Martin, BB Minkoff, MT Veling, I Lancaster, J Liu, JD Trimarco, B Bushnell, A Lipzen, EA Wood, MR Sussman, C Pennacchio, MM Cox. (2020) Physiology of Highly Radioresistant Escherichia coli After Experimental Evolution for 100 Cycles of Selection. Front Microbiol. 11:582590. DOI: 10.3389/fmicb.2020.582590 [PDF] Corrigendum DOI: 10.3389/fmicb.2020.617806

Henrikus, SS, C Henry, AE McGrath, S Jergic, JP McDonald, Y Hellmich, ST Bruckbauer, ML Ritger, ME Cherry, EA Wood, PT Pham, MF Goodman, R Woodgate, MM Cox, AM van Oijen, H Ghodke, A Robinson. (2020) Single-molecule live-cell imaging reveals RecB-dependent function of DNA polymerase IV in double strand break repair. Nucleic Acids Res, 48(15):8490-8508. DOI: 10.1093/nar/gkaa597

Romero, ZJ, SH Chen, T Armstrong, EA Wood, A van Oijen, A Robinson, MM Cox. (2020) Resolving toxic DNA repair intermediates in every E. coli replication cycle: critical roles for RecG, Uup and RadD. Nucleic Acids Res, 48(15):8445-8460. DOI: 10.1093/nar/gkaa579

Dubiel, K, C Henry, LM Spenkelink, AG Kozlov, EA Wood, S Jergic, NE Dixon, AM van Oijen, MM Cox, TM Lohman, SJ Sandler, JL Keck. (2020) Development of a Single-Stranded DNA-binding Protein Fluorescent Fusion Toolbox. Nucleic Acids Res, 48(11):6053-6067. DOI: 10.1093/nar/gkaa320

Romero, ZJ, TJ Armstrong, SS Henrikus, SH Chen, DJ Glass, AE Ferrazzoli, EA Wood, S Chitteni-Pattu, AM van Oijen,
ST Lovett, A Robinson, MM Cox. (2020) Frequent Template Switching in Postreplication Gaps: Suppression of Deleterious Consequences by the Escherichia Coli Uup and RadD Proteins. Nucleic Acids Research, 48, 212-230. DOI: 10.1093/nar/gkz960

Ghodke, H, BP Paudel, JS Lewis, S Jergic, K Gopall, ZJ Romero, EA Wood, R Woodgate, MM Cox and AM van Oijen. (2019) Spatial and temporal organization of RecA in the Escherichia coli DNA-damage response. eLife. 8 pii: e42761. DOI: 10.7554/eLife.42761.

Bruckbauer, ST, JD Trimarco, J Martin, B Bushnell, KA Senn, W Schackwitz, A Lipzen, M Blow, EA Wood, WS Culberson, C Pennacchio and MM Cox. (2019) Experimental evolution of extreme resistance to ionizing radiation in Escherichia coli after 50 cycles of selection. J Bacteriology. 201(8). pii: JB.00784-18. DOI: 10.1128/JB.00784-18.

Bruckbauer, ST, JD Trimarco, C Henry, EA Wood, JR Battista, and MM Cox. (2019) A variant of the Escherichia coli anaerobic transcription factor FNR exhibiting diminished promoter activation function enhances ionizing radiation resistance. PLoS One, 14(1): e0199482. DOI: 10.1371/journal.pone.0199482.

Henrikus, SS, C Henry, H Ghodke, EA Wood, N Mbele, R Saxena, U Basu, AM van Oijen, MM Cox, and A Robinson. (2019) RecFOR epistasis group: RecF and RecO have distinct localisations and functions in Escherichia coli. Nucleic Acids Research, 47, 2946-2965. DOI: 10.1093/nar/gkz003

Henrikus SS, Wood EA, McDonald JP, Cox MM, Woodgate R, Goodman MF, van Oijen AM, Robinson A. (2018) DNA polymerase IV primarily operates outside of DNA replication forks in Escherichia coli. PLoS Genet. 14(1):e1007161. doi: 10.1371/journal.pgen.1007161.

Lewis, JS, LS Spenkelink, S Jergic, EA Wood, E Monachino, NP Horan, KE Duderstadt, MM Cox, A Robinson, NE Dixon and AM van Oijen. (2017) Single-molecule visualization of fast polymerase turnover in the bacterial replisome. eLife 2017;6:e23932 pii: e23932. doi: 10.7554/3Life.23932.

Bakhlanova, IV, AV Dudkina, EA Wood, V Lanzov, MM Cox and DM Baitin (2016) DNA metabolism in balance: rapid loss of a RecA-based hyperrec phenotype. PLoS One. 11(4):e0154137. doi: 10.1371/journal.pone.0154137.

Robinson, A, JP McDonald, VEA Caldas, M Patel, EA Wood, CM Punter, H Ghodke, MM Cox, R Woodgate, MF Goodman, AM van Oijen (2015) Regulation of Mutagenic DNA Polymerase V Activation in Space and Time. PLoS Genetics. 11(8):e1005482. doi: 10.1371/journal.pgen.1005482.

Kim, T, S Chitteni-Pattu, BL Cox, EA Wood, SJ Sandler and MM Cox (2015) Directed evolution of RecA variants with enhanced capacity for conjugational recombination. PLoS Genetics. 11(6):e1005278. doi: 10.1371/journal.pgen.1005278

Chen, SH, RT Byrne, EA Wood and MM Cox (2015) Escherichia coli radD (yejH) gene: a novel function involved in radiation resistance and double-strand break repair. Mol Microbiol. 95, 754-768.

Byrne, RT, SH Chen, EA Wood, EL Cabot and MM Cox (2014) Escherichia coli Genes and Pathways Involved in Surviving Extreme Exposure to Ionizing Radiation. J Bact. 196, 3534-3545.

Byrne, RT, AJ Klingele, EL Cabot, WS Schackwitz, JA Martin, J Martin, Z Wang, EA Wood, C Pennacchio, LA Pennacchio, NT Perna, JR Battista and MM Cox (2014) Evolution of Extreme Resistance to Ionizing Radiation via Genetic Adaptation of DNA Repair. eLIFE. 3:e01322

Harris, D.R, S.V. Pollock, E.A. Wood, R.J. Goiffon, A.J. Klingele, E.L. Cabot, W. Schackwitz, J. Martin, J. Eggington, T.J. Durfee, C.M. Middle, J.E. Norton, M.C. Popelars, H. Li, S.A. Klugman, L.L. Hamilton, L.B. Bane, L. Pennacchio, T.J. Albert, N.T. Perna, M.M. Cox, and J.R. Battista. (2009). Directed evolution of ionizing radiation resistance in Escherichia coli. J. Bact. 191, 5240-5252.

Norais, C.A., S. Chitteni-Patu, E.A. Wood, R.B. Inman, and M.M. Cox. (2009). DdrB protein, an alternative Deinococcus radiodurans SSB induced by ionizing radiation. J. Biol. Chem. 284, 21402-21411.

Cox, J.M., H. Li, E.A. Wood, S. Chitteni-Pattu, R.B. Inman, and M.M. Cox. (2008). Defective dissociation of a “slow” RecA mutant protein imparts an Escherichia coli growth defect. J. Biol. Chem., 283, 24909-24921.

Eggington, J.M., Haruta., N., Wood, E.A., and Cox, M.M. (2004). The single-stranded DNA-binding protein of Deinococcus radiodurans. BMC Microbiology, 4, Art. No. 2.

Lusetti, S.L., Wood, E.A., Fleming, C.D., Modica, M.J., Korth, J., Abbott, L., Dwyer, D.W., Roca, A.I., Inman, R.B. and Cox, M.M. (2003) C terminal deletions of the Escherichia coli RecA protein: Characterization of in vivo and in vitro effects. J. Biol. Chem. 278, 16372-16380

Kim, J.-I., Sharma, A.K., Abbott, S.N., Wood, E.A., Dwyer, D.W., Jambura, A., Minton, K.W., Inman, R.B., Daly, M.J., and Cox, M.M. (2002). RecA protein from the extremely radioresistant bacterium Deinococcus radiodurans: Expression, purification, and characterization. J. Bact., 184, 1649-1660

Huang, L.-C., Wood, E.A. and Cox, M.M. (1997). Convenient and reversible site-specific targeting of exogenous DNA into a bacterial chromosome using the FLP recombinase: the FLIRT system. J. Bact. 179, 6076-6083

Iype, L. E., Wood, E. A., Inman, R. B. and Cox, M. M. (1994). RuvA and RuvB proteins facilitate the bypass of heterologous DNA insertions during RecA protein-mediated DNA strand exchange. J. Biol. Chem. 269, 24967-24978

Huang, L.-C., Wood, E. A. and Cox, M. M. (1991). A bacterial model system for chromosomal targeting. Nucleic Acids Res. 19, 443-448

Meyer-Leon, L., Gates, C. A., Attwood, J. M., Wood, E. A. and Cox, M. M. (1987). Purification of the FLP site-specific recombinase by affinity chromatography and re-examination of basic properties of the system. Nucleic Acids Res. 15, 6469-6488

Gates, C. A., Meyer-Leon, L., Attwood, J. M., Wood, E. A. and Cox, M. M. (1987). Purification of FLP recombinase using sequence-specific DNA affinity chromatography. In Protein Purification: Micro to Macro, R. Burgess, ed. (Alan R. Liss), UCLA Symp. Mol. Cell. Biol., New Ser., 68, 197-206

Senecoff, J. F., Bruckner, R C., Meyer-Leon, L., Gates, C. A., Wood, E., Umlauf, S. W., Attwood, J. M. and Cox, M. M. (1986). Site-specific recombination promoted in vitro by the FLP protein of the yeast two-micron plasmid. In Extrachromosomal Elements in Lower Eukaryotes, R. B.Wickner et al., eds. (Plenum Press), pp. 397-405